Jump to content

PubMed RSS Feed - - Non-invasive in vivo detection and quantification of Demodex mites by confocal laser scanning microscopy.


rss

Recommended Posts

Non-invasive in vivo detection and quantification of Demodex mites by confocal laser scanning microscopy.

Br J Dermatol. 2012 Jun 20;

Authors: Sattler EC, Maier T, Hoffmann VS, Hegyi J, Ruzicka T, Berking C

Abstract
Background:  In many Demodex-associated skin diseases Demodex mites are present in abundance and seem to be at least partially pathogenic. So far all diagnostic approaches such as scraping or standardized superficial skin biopsy are (semi-) invasive and may cause discomfort to the patient. Aim of this study was to see whether confocal laser scanning microscopy (CLSM) - a non-invasive method for the visualization of superficial skin layers - was able to detect and quantify Demodex folliculorum in facial skin of patients with rosacea. Material and Methods:  25 patients (34-79 years of age) with facial rosacea and 25 age- and gender-matched normal controls were examined by CLSM. Mosaics of 8x8 mm and 5x5 mm were created by scanning horizontal layers of lesional skin and quantification of the mites per follicle and per area as well as of the follicles per area was performed. Results:  In all patients Demodex folliculorum could be detected by CLSM and presented as roundish or lengthy cone-shaped structures. CLSM allowed the quantification of Demodex mites and showed significant differences (p-value < 0.0001): The mean number of mites was 165.4 per 8x8 mm area and 94.2 per 5x5 mm area in the patients compared to 34.7 and 22.4, respectively, in the controls. The corresponding mean number of mites per follicle was 0.7 and 0.8, respectively, in the patients and 0.1 and 0.2, respectively, in the controls. Conclusion:  With the help of CLSM it is possible to non-invasively detect, image and quantify Demodex mites in facial skin of patients with rosacea.

PMID: 22716072 [PubMed - as supplied by publisher]

http://www.ncbi.nlm.nih.gov/PubMed/22716072?dopt=Abstract = URL to article

Link to comment
Share on other sites

Join the conversation

You can post now and register later. If you have an account, sign in now to post with your account.
Note: Your post will require moderator approval before it will be visible.

Guest
Unfortunately, your content contains terms that we do not allow. Please edit your content to remove the highlighted words below.
Reply to this topic...

×   Pasted as rich text.   Paste as plain text instead

  Only 75 emoji are allowed.

×   Your link has been automatically embedded.   Display as a link instead

×   Your previous content has been restored.   Clear editor

×   You cannot paste images directly. Upload or insert images from URL.

×
×
  • Create New...

Important Information

Terms & Rules