rss Posted January 3, 2013 Report Share Posted January 3, 2013 Azelaic acid reduced senescence-like phenotype in photo-irradiated human dermal fibroblasts: possible implication of PPARÎ³. Exp Dermatol. 2013 Jan;22(1):41-7 Authors: Briganti S, Flori E, Mastrofrancesco A, Kovacs D, Camera E, Ludovici M, Cardinali G, Picardo M Abstract Azelaic acid (AzA) has been used for the treatment for inflammatory skin diseases, such as acne and rosacea. Interestingly, an improvement in skin texture has been observed after long-time treatment with AzA. We previously unrevealed that anti-inflammatory activity of AzA involves a specific activation of PPARÎ³, a nuclear receptor that plays a relevant role in inflammation and even in ageing processes. As rosacea has been considered as a photo-aggravated disease, we investigated the ability of AzA to counteract stress-induced premature cell senescence (SIPS). We employed a SIPS model based on single exposure of human dermal fibroblasts (HDFs) to UVA and 8-methoxypsoralen (PUVA), previously reported to activate a senescence-like phenotype, including long-term growth arrest, flattened morphology and increased synthesis of matrix metalloproteinases (MMPs) and senescence-associated Î²-galactosidase (SA-Î²-gal). We found that PUVA-treated HDFs grown in the presence of AzA maintained their morphology and reduced MMP-1 release and SA-Î²-galactosidase-positive cells. Moreover, AzA induced a reduction in ROS generation, an up-modulation of antioxidant enzymes and a decrease in cell membrane lipid damages in PUVA-treated HDFs. Further evidences of AzA anti-senescence effect were repression of p53 and p21, increase in type I pro-collagen and abrogation of the enhanced expression of growth factors, such as HGF and SCF. Interestingly, PUVA-SIPS showed a decreased activation of PPARÎ³ and AzA counteracted this effect, suggesting that AzA effect involves PPARÎ³ modulation. All together these data showed that AzA interferes with PUVA-induced senescence-like phenotype and its ability to activate PPAR-Î³ provides relevant insights into the anti-senescence mechanism.PMID: 23278893 [PubMed - in process] http://www.ncbi.nlm.nih.gov/PubMed/23278893?dopt=Abstract = URL to article Link to comment Share on other sites More sharing options...
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