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Demodex quantification methods: Limitations of Confocal Laser Scanning Microscopy (CLSM).

Br J Dermatol. 2013 Feb 16;

Authors: Lacey N, Forton FM, Powell FC

Abstract
We read with interest the recent paper by Sattler et al.,(1) describing the quantification of Demodex mites by confocal laser scanning microscopy (CLSM). Their work is interesting but we feel there are limitations to the use of this method to accurately detect absolute numbers of mites in human skin. Because the role of Demodex in normal or diseased skin is unknown,(2) their possible role in modulation of immune reactivity of the follicle(3) and an association between the degree of mite infestation and the development of rosacea,(4-6) accurate reproducible in vivo methods of quantifying mites is becoming increasingly important.

PMID: 23414055 [PubMed - as supplied by publisher]

http://www.ncbi.nlm.nih.gov/PubMed/23414055?dopt=Abstract = URL to article

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