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Non-invasive in vivo detection and quantification of Demodex mites by confocal laser scanning microscopy Reply from authors.

Br J Dermatol. 2013 Feb 20;

Authors: Sattler EC, Maier T, Hoffmann VS, Ruzicka T, Berking C

Abstract
we thank Lacey et al. for their interesting and very welcome thoughts concerning our article on the usefulness of confocal laser scanning microscopy (CLSM) in the quantification of demodex mites and for their excellent pictures of Demodex mites seen under the light microscope after extraction using the standardized skin surface biopsy (SSSB) method. As a center specialized in acne and rosacea we are also using SSSB routinely as the standard method for demodex quantification and have gathered ample experience within the past decades. We agree that mainly Demodex folliculorum is detected with both SSSB and especially with CLSM (with a penetration depths limited to about 200-300 μm), since Demodex folliculorum is usually located at the upper part of the infundibulum of the hair follicle, while Demodex brevis is usually seen at the lower portion close to the sebaceous glands.

PMID: 23421742 [PubMed - as supplied by publisher]

http://www.ncbi.nlm.nih.gov/PubMed/23421742?dopt=Abstract = URL to article

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