Jump to content

PubMed RSS Feed - -Reproducing the scalp microbiota community: co-colonization of a 3D reconstructed human epidermis with C. acnes and M. restricta.


rss

Recommended Posts

Related Articles

Reproducing the scalp microbiota community: co-colonization of a 3D reconstructed human epidermis with C. acnes and M. restricta.

Int J Cosmet Sci. 2021 Jan 20;:

Authors: Meloni M, Balzaretti S, Collard N, Desaint S, Laperdrix C

Abstract
OBJECTIVE: A 3D reconstructed human epidermis (RHE) model colonized with specific microbial strains was developed to model the complex interactions between strains of the human scalp hair.
METHODS: RHE was colonized with Cutibacterium acnes and Malassezia restricta for 72h. The epidermal model was characterized in term of morphology, using immune-labeling targeting biomarkers for barrier structure, proliferation, differentiation and antimicrobial defense. The barrier function was assessed by Trans Epithelial Electrical Resistance (TEER) measurements. In order to study the microorganisms on the epidermal model, viable counts and phenotype ultrastructure analysis were performed by scanning electron microscopy (SEM).
RESULTS: The RHE colonized with C. acnes did not lead to severe modifications of the physiological barrier integrity and viability, though it shows aggregates. M. restricta formed large aggregates by a close interaction with the RHE, thus causing both a strong decrease in barrier function and structure degradation and an increased Human Beta Defensin 2 (HBD2) expression. The co-colonized model resulted in barrier depletion, but the overall damage was less severe, respecting the single colonization with M. restricta. The developed 'scalp model' allowed to identify morphological modifications, leading to uncontrolled epidermal renewal and anti-microbial response towards the microbial colonization.
CONCLUSION: This study shows a pre-clinical model that recapitulates the interactions that can occur between site specific microbial strains and keratinocytes in dandruff condition. The model can be applied to assess ingredients and products' mechanism of action.

PMID: 33469935 [PubMed - as supplied by publisher]

{url} = URL to article

Link to comment
Share on other sites

Join the conversation

You can post now and register later. If you have an account, sign in now to post with your account.
Note: Your post will require moderator approval before it will be visible.

Guest
Reply to this topic...

×   Pasted as rich text.   Paste as plain text instead

  Only 75 emoji are allowed.

×   Your link has been automatically embedded.   Display as a link instead

×   Your previous content has been restored.   Clear editor

×   You cannot paste images directly. Upload or insert images from URL.

×
×
  • Create New...