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Increased expression of IL-33 in rosacea skin and UVB-irradiated and LL-37-treated HaCaT cells.

Exp Dermatol. 2018 Jun 06;:

Authors: Suhng E, Kim BH, Choi YW, Choi HY, Cho H, Byun JY

Abstract
BACKGROUND: Rosacea is one of the most common dermatoses of adults. Although the detailed pathophysiology remains unknown, it is thought that rosacea is caused by a consistently aberrant, innate immune response, and that LL-37 plays an important role. However, involvement of the inflammatory cytokine IL-33 has not yet been studied.
OBJECTIVES: We explored the role played by IL-33 in the pathophysiology of rosacea.
METHODS: First, we immunohistochemically evaluated the expression of IL-33 and its receptor (ST2) in rosacea skin. Second, we exposed HaCaT cells to ultraviolet B (UVB) irradiation in the presence or absence of LL-37, and measured the expression of proinflammatory cytokines including IL-33. We also analyzed VEGF (vascular endothelial growth factor) mRNA expression and protein release after co-stimulation of HaCaT cells by LL-37 and IL-33.
RESULTS: Immunohistochemically, IL-33 expression was enhanced in the skin of rosacea patients, especially with erythematotelangiectatic subtype. In vitro, UVB and LL-37 synergistically increased mRNAs expression of proinflammatory cytokines, especially IL-33 and IL-1β. IL-33 protein release was also synergistically increased by LL-37 and UVB treatment. LL-37 and IL-33 stimulated VEGF mRNA expression and VEGF release from HaCaT cells.
CONCLUSIONS: Our findings suggest that rosacea skin with abundant LL-37 may robustly produce and release IL-33 when exposed to UV radiation. IL-33 may participate in the angiogenesis and vasodilation of rosacea skin by enhancing VEGF release. This article is protected by copyright. All rights reserved.

PMID: 29873850 [PubMed - as supplied by publisher]

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