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Evaluation of Demodex mite viability using motility and scattered light intensity.

Exp Appl Acarol. 2019 Apr 19;:

Authors: Gatault S, Foley R, Shiels L, Powell FC

Demodex mites have been suggested to have a role in various cutaneous and ocular disorders pathogenesis, such as rosacea or blepharitis. Evaluation of potential treatments with anti-Demodex effects is difficult because the viability of living mites needs to be evaluated during their exposure to the agent being tested. Mite viability is currently based solely on their observed movement. However, this method of assessing viability has significant limitations as mites may be resting, immobile or paralysed at any given observation point giving the observer a false impression of the organism's death. To overcome this limitation we evaluated a new quantitative method of evaluating the viability of Demodex mites by using scattered light intensity (SLI). We demonstrated that when combined with observation of mite motility, SLI provided increased accuracy of the evaluation of viability of mites being studied. This new viability assay will help address the technical challenges of mite viability experiments. Accurate evaluation of mite viability will enhance mite biology research and allow for more accurate in vitro toxicity assays of proposed anti-mite agents.

PMID: 31001698 [PubMed - as supplied by publisher]

{url} = URL to article • JID article

Scroll to the subheading. [0ther] Tools to Detect or Quantify Demodex Density Counts, in this post or 
Methods for Quantifying Demodex Mites

"Here we report a novel scattered light imaging (SLi) technique that allows label-free NPs to be monitored using a conventional confocal microscope." 
J. Am. Chem. Soc. 2019, 141, 36, 14043–14047
Scattered Light Imaging Enables Real-Time Monitoring of Label-Free Nanoparticles and Fluorescent Biomolecules in Live Cells
Fengbang Wang, Bolei Chen, Bing Yan, Yongguang Yin, Ligang Hu, Yong Liang, Maoyong Song, Guibin Jiang


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